Detection of sulfur-containing compounds in control and cadmium-exposed rat organs by high-performance liquid chromatography--vacuum-ultraviolet inductively coupled plasma-atomic emission spectrometry (HPLC-ICP) |
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| Authors: | H Sunaga E Kobayashi N Shimojo K T Suzuki |
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| Affiliation: | 1. National Institute for Environmental Studies, Yatabe, Tsukuba, Ibaraki 305, Japan;2. Institute of Environmental Sciences, University of Tsukuba, Niihari, Ibaraki 305, Japan;1. Department of Physics, Karnatak Science College, Karnatak University, Dharwad 580001, Karnataka, India;1. State Key Laboratory of Microbial Metabolism, and Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China;2. State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China;3. Jiangxi Key Laboratory of Mining & Metallurgy Environmental Pollution Control, Jiangxi University of Science and Technology, Ganzhou, Jiangxi, 341000, China;1. Department of Ocean Sciences, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan;2. National Research Institute of Fisheries Science, Yokohama, 236-8648, Japan;1. Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia;2. Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia;3. ICTM Department of Chemistry, University of Belgrade, Belgrade, Serbia;4. Institute of Occupational Health of Serbia “Dr Dragomir Karajović”, Belgrade, Serbia;5. Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research “Vinca”, University of Belgrade, Serbia |
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| Abstract: | Sulfur-containing compounds in biological samples were separated by high-performance gel permeation chromatography and detected with a vacuum-ultraviolet inductively coupled plasma-atomic emission spectrometer. Distribution profiles of sulfur in the supernatants of liver, kidney, spleen, lung, and pancreas of control and cadmium-exposed rats were determined along with cadmium, copper, iron, phosphorus, and zinc profiles. Changes in sulfur distribution were induced by cadmium exposure not only in the metallothionein fraction, but also in the high-molecular-weight protein fraction, indicating the effect of cadmium exposure on diverse endogenous sulfur-containing compounds. Glutathione and taurine also were detected simultaneously as distinct peaks. |
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