首页 | 本学科首页   官方微博 | 高级检索  
     


Simple and versatile selection of Arabidopsis transformants
Authors:M. Hadi  E. Kemper  E. Wendeler  B. Reiss
Affiliation:1.Max-Planck-Institut fuer Zuechtungsforschung, Department Schell, Carl-von-Linne-Weg 10, 50829 Cologne, Germany,
Abstract:The selection of large amounts of seeds of Arabidopsis thaliana obtained after in planta transformation under sterile conditions and in tissue culture is relatively labour-intensive, time-consuming and expensive. We describe here an alternative selection procedure that uses regular greenhouse conditions and functions with a wide variety of selective agents. The selection system is based on the inert growth substrate Grodan and was intensively tested for the selection of transgenic Arabidopsis seeds with kanamycin, hygromycin, BASTA, sulfadiazine and methotrexate. However, the system has the capacity to be applicable for more selective agents and to a wider range of plant species. For Arabidopsis, the effectiveness of selective agents is comparable to or better than that obtained with tissue culture procedures.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号