Determination of anti-Aspergillus activity of antifungal agents based on the dynamic growth rate of a single hypha |
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| Authors: | Kibong Oh Hideaki Matsuoka Yasuyuki Nemoto Osao Sumita Kosuke Takatori Hiroshi Kurata |
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| Institution: | (1) Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16, Nakamachi, 184 Koganei, Tokyo, Japan;(2) Research and Development Division, Bio-Giken Inc., 1-5-8, Iwamotocho, 101 Chiyodaku, Tokyo, Japan;(3) Hatano Research Institute, Food and Drug Safety Center, 729-5, Ochiai, 257 Hadano, Kanagawa, Japan;(4) The Tokyo Kembikyoin Foundation, 4-8-32, Kudanminami, 102 Chiyodaku, Tokyo, Japan |
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| Abstract: | The dynamic growth rate of a single hypha of Aspergillus niger was analysed using an automatic system. A colony of A. niger was in contact with saline, saline containing an antifungal agent, and flushing saline, in sequence. The growth rate of a test hypha selected arbitrarily from the colony responded dynamically to the antifungal agent. The minimum concentration that caused the complete inhibition of hyphal growth was defined as the minimum inhibitory concentration (MIC). The MIC values obtained were compared with those determined by conventional methods based on increasing rate of colony diameter or dry matter weight. |
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