Expression of TrkB and BDNF in human cochlea-an immunohistochemical study |
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Authors: | Liu Wei Kinnefors Anders Boström Marja Rask-Andersen Helge |
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Institution: | (1) Department of Otolaryngology, Uppsala University Hospital, 75185 Uppsala, Sweden;(2) Department of Surgical Sciences, Section of Otolaryngology, Uppsala University Hospital, SE-751 85 Uppsala, Sweden |
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Abstract: | Surgical human cochlear specimens were obtained during the removal of large posterior cranial fossa meningioma by a transcochlear
approach in which the cochlea was removed for maximal exposure of the tumor and protection of important structures, such as
the brainstem, cranial nerves, and pivotal blood vessels. The cochlear tissue was fixed and cryo-sectioned for tyrosine kinase
receptor B (TrkB) and brain-derived neurotrophic factor (BDNF) immunohistochemistry. TrkB receptor protein was expressed in
both neuronal somata and the processes of human spiral ganglion neurons (SGNs). In the human organ of Corti, TrkB immunoreactivity
was mainly present in nerve fibers underneath outer hair cells. BDNF expression was found neither in the organ of Corti nor
in the spiral ganglion of human cochlea. For antibody specificity and for control and comparative purposes, TrkB immunocytochemistry
was performed in primary cultures of cochlear neuron/glia from adult guinea pig. Confocal laser scanning microscopy showed
that TrkB was homogeneously distributed in the cytoplasm of both neuronal somata and axons. Knowledge of the expression of
TrkB receptor in human cochlea should help to determine the target structures for neuron preservation in hearing-impaired
patients. Our results indicate that the regeneration of SGNs under pathological conditions can be enhanced with BDNF/TrkB-based
pharmaceutical or genetic strategies. |
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