Proteome analysis of microtubule-associated proteins and their interacting partners from mammalian brain |
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Authors: | Frank?Kozielski Tahira?Riaz Salvatore?DeBonis Christian?J?Koehler Mario?Kroening Isabel?Panse Margarita?Strozynski Ian?M?Donaldson Email author" target="_blank">Bernd?ThiedeEmail author |
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Institution: | (1) The Beatson Institute for Cancer Research, Switchback Road, Bearsden, Glasgow, G61 1BD, Scotland, UK;(2) The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, 0349 Oslo, Norway;(3) Institut de Biologie Structurale (CEA-CNRS-UJF), 41 rue Jules Horowitz, 38027 Grenoble Cedex 01, France;(4) The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, P.O. Box 1125, Blindern, 0317 Oslo, Norway; |
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Abstract: | The microtubule (MT) cytoskeleton is essential for a variety of cellular processes. MTs are finely regulated by distinct classes
of MT-associated proteins (MAPs), which themselves bind to and are regulated by a large number of additional proteins. We
have carried out proteome analyses of tubulin-rich and tubulin-depleted MAPs and their interacting partners isolated from
bovine brain. In total, 573 proteins were identified giving us unprecedented access to brain-specific MT-associated proteins
from mammalian brain. Most of the standard MAPs were identified and at least 500 proteins have been reported as being associated
with MTs. We identified protein complexes with a large number of subunits such as brain-specific motor/adaptor/cargo complexes
for kinesins, dynein, and dynactin, and proteins of an RNA-transporting granule. About 25% of the identified proteins were
also found in the synaptic vesicle proteome. Analysis of the MS/MS data revealed many posttranslational modifications, amino
acid changes, and alternative splice variants, particularly in tau, a key protein implicated in Alzheimer’s disease. Bioinformatic
analysis of known protein–protein interactions of the identified proteins indicated that the number of MAPs and their associated
proteins is larger than previously anticipated and that our database will be a useful resource to identify novel binding partners. |
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