The use of lectins for determination of absolute configurations of small amounts of sugars eluted from chromatograms |
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| Authors: | V Horejsí M Tichá T Trnka |
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| Affiliation: | 1. Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Vídeňská 1083, 142 20 Prague 4, Czechoslovakia;1. Department of Biochemistry, Charles University, Albertov 2030, Prague 2, Czechoslovakia;2. Department of Organic Chemistry, Charles University, Albertov 2030, Prague 2, Czechoslovakia |
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| Abstract: | A simple procedure for the determination of the absolute configuration (i.e., assignment to the D- or L-enantiomeric series) of glucose, mannose, galactose, fucose, arabinose, and rhamnose is described, based on inhibition by these sugars of 125I-labeled lectin binding to the glycoconjugates immobilized on the wells of plastic microculture plates. The method works well with 10 to 100-micrograms amounts of the sugars isolated after paper chromatography of the glycoprotein or polysaccharide hydrolysates. |
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