Sodium thioglycollate enhances pollen germination and pollen tube elongation in cruciferous species |
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Authors: | Lidia S Watrud Jim Brewer Tamotsu Shiroyama Bonnie M Smith George A King |
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Institution: | (1) Western Ecology Division, US Environmental Protection Agency National Health and Environmental Effects Research Laboratory, 200 SW 35th St, Corvallis, OR 97333, USA;(2) Dynamac Corporation, 200 SW 35th St, Corvallis, OR 97333, USA |
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Abstract: | Sodium thioglycollate is a reducing agent used in microbiological growth media to enhance the growth of anerobic, microaerophilic,
and facultative organisms, and in eukaryotic tissue extraction buffers to inhibit damaging oxidative reactions. Sodium thioglycollate
was added to a semi-solid pollen germination medium to evaluate its effects on in vitro pollen germination and pollen tube elongation, based on the assumption that conditions within stylar tissues are less aerobic
than in ambient conditions. We observed significant increases in the percent germination and pollen tube elongation of both
crop and weedy mustard family species, on a medium containing 2.2 mM sodium thioglycollate. This suggests that sodium thioglycollate
may be a useful amendment to semi-solid media and to solutions that are used to study pollen vigor, physiology, or gene expression,
and to bioassay sensitivities of different species or genotypes to diverse physical and chemical factors. |
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