Evidence that the zymogen of phospholipase A2 binds to a negatively charged lipid-water interface |
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Authors: | J J Volwerk P C Jost G H de Haas O H Griffith |
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Institution: | 1. Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 U.S.A.;2. Biochemical Laboratory, State University of Utrecht, Padualaan 8, 3508 TB-Utrecht The Netherlands |
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Abstract: | Evidence is presented that the zymogen of porcine pancreatic phospholipase A2 (prophospholipase A2) interacts with a lipid-water interface provided that the interface has a net negative surface charge. Fluorescence spectroscopy and non-equilibrium gel filtration indicate that binding of prophospholipase A2 (proPLA) to mixed detergent micelles is dependent on the presence of an anionic detergent. Prophospholipase binding is accompanied by a change in the environment of the single tryptophan residue qualitatively similar to that observed when the active enzyme, phospholipase A2 (PLA), binds to micelles. In addition, the rate of tryptic activation of prophospholipase is significantly reduced in the presence of negatively-charged mixed micelles, whereas no change in rate occurs when neutral micelles are present. These observations suggest that the lack of catalytic activity of the zymogen toward organized substrates carrying a negative surface charge cannot be explained by a failure to bind at the lipid-water interface. |
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Keywords: | zymogen interfacial binding surface charge PLA proPLA SDS sodium dodecyl sulfate CMC critical micellar concentration TPCK tosyl-phenylalanine chloromethylketone |
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