Recombinational hotspot specific to female meiosis in the mouse major histocompatibility complex |
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Authors: | Toshihiko Shiroishi Naoto Hanzawa Tomoko Sagai Masahiro Ishiura Takashi Gojobori Michael Steinmetz Kazuo Moriwaki |
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Affiliation: | (1) Department of Cell Genetics, National Institute of Genetics, Yata-1111, 411 Mishima, Japan;(2) Division of Cell Fusion, Department of Cell Biology, National Institute for Basic Biology, Nishigonaka 38, 444 Myodaiji-cho, Okazaki, Japan;(3) Department of Evolutionary Genetics, National Institute of Genetics, Yata-1111, 411 Mishima, Japan;(4) Central Research Units, F. Hoffmann-La Roche & Co. Ltd., CH-4002 Basel, Switzerland;(5) Present address: Institute for Marine Biotechnology, Hongoh, Bunkyou-ku, 113 Tokyo, Japan |
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Abstract: | Thewm7 haplotype of the major histocompatibility complex (MHC), derived from the Japanese wild mouseMus musculus molossinus, enhances recombination specific to female meiosis in theK/A interval of the MHC. We have mapped crossover points of fifteen independent recombinants from genetic crosses of thewm7 and laboratory haplotypes. Most of them were confined to a short segment of approximately 1 kilobase (kb) of DNA between theA 3 andA 2 genes, indicating the presence of a female-specific recombinational hotspot. Its location overlaps with a sex-independent hotspot previously identified in theMus musculus castaneus CAS3 haplotype. We have cloned and sequenced DNA fragments surrounding the hotspot from thewm7 haplotype and the corresponding regions from the hotspot-negative B10.A and C57BL/10 strains. There is no significant difference between the sequences of these three strains, or between these and the published sequences of the CAS3 and C57BL/6 strains. However, a comparison of this A 3/A 2 hotspot with a previously characterized hotspot in theE gene revealed that they have a very similar molecular organization. Each hotspot consists of two elements, the consensus sequence of the mouse middle repetitive MT family and the tetrameric repeated sequences, which are separated by 1 kb of DNA.The nucleotide sequence data reported in this paper have been submitted to the DNA Data Bank of Japan nucleotide sequence database and have been assigned the accession numbers d90007-9.Offprint requests to: T. Shiroishi. |
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