The amino-terminal region of major capsid protein P3 is essential for self-assembly of single-shelled core-like particles of Rice dwarf virus |
| |
Authors: | Hagiwara Kyoji Higashi Takahiko Miyazaki Naoyuki Naitow Hisashi Cheng R Holland Nakagawa Atsushi Mizuno Hiroshi Tsukihara Tomitake Omura Toshihiro |
| |
Institution: | Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan. |
| |
Abstract: | The core protein P3 of Rice dwarf virus constructs asymmetric dimers, one of which is inserted by the amino-terminal region of another P3 protein. The P3 proteins with serial amino-terminal deletions, expressed in a baculovirus system, formed particles with gradually decreasing stability. The capacity for self-assembly disappeared when 52 of the amino-terminal amino acids had been deleted. These results demonstrated that insertion of the amino-terminal arm of one P3 protein into another appears to play an important role in stabilizing the core particles. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|