Site-specific DNA excision in transgenic rice with a cell-permeable Cre recombinase |
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Authors: | Ming-Xia Cao Jian-Qiu Huang Quan-Hong Yao Sheng-Jun Liu Cheng-Long Wang Zhi-Ming Wei |
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Affiliation: | (1) National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, People's Republic of China;(2) Agrobiotech Research Center, Shanghai Academy of Agricultural Sciences, 201106 Shanghai, People's Republic of China |
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Abstract: | The removal of selected marker genes from transgenic plants is necessary to address biosafety concerns and to carry out further experiments with transgenic organisms. In the present study, the 12-amino-acid membrane translocation sequence (MTS) from the Kaposi fibroblast growth factor (FGF)-4 was used as a carrier to deliver enzymatically active Cre proteins into living plant cells, and to produce a site-specific DNA excision in transgenic rice plants. The process, which made cells permeable to Cre recombinase-mediated DNA recombination, circumvented the need to express Cre under spatiotemporal control and was proved to be a simple and efficient system to achieve marker-free transgenic plants. The ultimate aim of the present study is to develop commercial rice cultivars free from selected marker genes to hasten public acceptance of transgenic crops. |
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Keywords: | MTS cell-permeable Cre recombinase marker-free transgenic rice plants biosafety |
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