Selective photo-cross-linking of 2'-O-psoralen-conjugated oligonucleotide with RNAs having point mutations |
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Authors: | Higuchi Maiko Yamayoshi Asako Yamaguchi Takasei Iwase Reiko Yamaoka Tetsuji Kobori Akio Murakami Akira |
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Affiliation: | Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan. |
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Abstract: | It has been reported that point mutations in genes are responsible for various cancers and the selective regulation of the gene expression is an important issue to develop a new type of anticancer drugs. In this report, we present a new type of antisense molecule that photo-cross-links to an oligoribonucleotide having a point mutation site in a sequence specific manner. 2'-O-psoralen-conjugated adenosine was synthesized in four steps from adenosine and introduced in the middle of an oligodeoxyribonucleotide (2'-Ps-oligo). Compared with 5'-O-psoralen-conjugated oligodeoxyribonucleotide (5'-Ps-oligo), which has a psoralen at the 5'-end, 2'-Ps-oligo more selectively photo-cross-linked to a pyrimidine base of the site of alteration from purine to pyrimidine in the oligoribonucleotide. |
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