Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA |
| |
Authors: | Arie Rosner Roberto N. Bastos Haim Aviv |
| |
Affiliation: | (1) Department of Virology, Weizmann Institute of Science, Rehovot, Israel |
| |
Abstract: | A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids orS. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |