Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA |
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| Authors: | Arie Rosner Roberto N Bastos Haim Aviv |
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| Institution: | (1) Department of Virology, Weizmann Institute of Science, Rehovot, Israel |
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| Abstract: | A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids orS. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared. |
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