Introduction of tau Mutation into Cultured Rat1-R12 Cells by Gene Targeting,Using Recombinant Adeno-Associated Virus Vector |
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Authors: | Hiroko Shimada Kahori Numazawa Tsukasa Sasaki Nobumasa Kato Takashi Ebisawa |
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Affiliation: | (1) Department of Sleep Disorder Research (Alfressa), Graduate School of Medicine, University of Tokyo, Tokyo, Japan;(2) Health Service Center, University of Tokyo, Tokyo, Japan;(3) Department of Neuropsychiatry, Graduate School of Medicine, University of Tokyo, Tokyo, Japan;(4) Present address: Department of Physiology, Keio University School of Medicine, Tokyo, Japan;(5) Present address: Chugai Pharmaceutical Co., Ltd., Tokyo, Japan;(6) Present address: Department of Psychiatry, Showa University School of Medicine, Tokyo, Japan;(7) Present address: Department of Psychiatry, Tokyo Metropolitan Police Hospital, 4-22-1, Nakano, Nakano-ku, Tokyo 164-8541, Japan; |
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Abstract: | We aim to develop a cultured cell model, to serve as a system with which the altered circadian phenotypes produced by the clock gene variations could be studied in vitro. Tau mutation, which shortens the circadian period of hamsters and mice, was introduced into the CK1ε locus of cultured Rat1-R12 cells by gene targeting mediated by a recombinant adeno-associated virus (rAAV) vector. After transduction of Rat1-R12 cells with rAAV, about 0.14% of the drug-resistant cells underwent gene targeting at CK1ε locus. Of the three clones isolated, only one carried the targeted allele of tau mutation and two carried the targeted wild-type allele. The clone with the targeted tau mutant allele exhibited a significantly shorter circadian period compared to the clone with targeted wild-type allele. rAAV-mediated gene targeting in cultured somatic cells is a convenient and powerful tool for analyzing the phenotypic outcome of clock gene variations, and for elucidating the pathogenesis of the disorders associated with abnormal circadian rhythmicity. |
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Keywords: | Circadian rhythm Gene targeting Adeno-associated virus Clock gene Gene mutation |
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