Inhibition of amino acid transport in Bacillus subtilis by uncouplers of oxidative phosphorylation. |
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Authors: | T B Brummett G W Ordal |
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Affiliation: | Department of Biochemistry and School of Basic Medical Sciences, University of Illinois, Urbana, Illinois 61801 U.S.A. |
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Abstract: | The effect of three uncouplers of oxidative phosphorylation, trifluoromethoxycarbon-ylcyanidephenylhydrazone (FCCP), 3,3′,4′,5-tetrachlorosalicylanilide (TCSA), and pentachlorophenol (PCP), on transport of glycine and proline by Bacillus subtilis were examined. FCCP inhibited proline uptake uncompetitively, but glycine uptake competitively. TCSA inhibited proline uptake noncompetitively, but glycine uptake competitively. PCP inhibited proline uptake noncompetitively, but glycine uptake uncompetitively. The results indicate that these uncouplers inhibit amino acid transport by interacting at specific sites rather than by reducing any central supply of energy used to fuel metabolic processes. |
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Keywords: | To whom all correspondence should be addressed. |
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