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层孔菌产漆酶的摇瓶最适培养条件研究
引用本文:康从宝,赵建,李清心,曲音波,高培基. 层孔菌产漆酶的摇瓶最适培养条件研究[J]. 微生物学通报, 2002, 29(3): 42-45
作者姓名:康从宝  赵建  李清心  曲音波  高培基
作者单位:山东大学微生物技术国家重点实验室,济南,250100
基金项目:国家自然科学基金资助项目 (No. 2 0 0 770 1 5 ),华南理工大学制浆造纸工程国家重点实验室开放基金资助项目
摘    要:筛选到一株高产漆酶的层孔菌W-1,对其兴体产漆酶的培养条件进行了优化,确定了最适的碳源、氮源,并用正交试验确定了培养基中碳源和氮源的浓度。在最优化的培养条件下,培养7d后,W-1产生的漆酶活力可以达到7.1U/mL。采用补料的方法,可以得到大量的漆酶粗酶液。

关 键 词:层孔菌 漆酶 摇瓶 最适培养条件 白腐菌 正交试验
文章编号:0253-2654(2002)03-0042-04
修稿时间:2001-03-01

STUDY ON LIQUID CONDITIONS IN SHAKING FLASKS FOR LACCASE PRODUCTION BY A WHITE ROT FUNGI
KANG Cong-Bao ZHAO Jian LI Qing-Xin QU Yin-Bo GAO Pei-Ji. STUDY ON LIQUID CONDITIONS IN SHAKING FLASKS FOR LACCASE PRODUCTION BY A WHITE ROT FUNGI[J]. Microbiology China, 2002, 29(3): 42-45
Authors:KANG Cong-Bao ZHAO Jian LI Qing-Xin QU Yin-Bo GAO Pei-Ji
Abstract:A white-rot fungi Rigidoporus sp.W-1 which could produce laccase was isolated. The fermentation conditions in shaking flasks were investigated. The optimal carbon source was wheat bran and (NH 4) 2SO 4 was the optimal nitrogen source. The components of the medium were optimized by orthogonal experiment. When W-1 was cultured under the optimum conditions, the activity of laccase could get to 7.1U/mL in 7 days.A great amount of crude laccase could be obtained by adding fresh medium to the 7 days old mycelium.
Keywords:White rot fungus   Laccase   Orthogonal experiment  
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