Molecular cloning and functional analysis of an organ-specific expressing gene coding for farnesyl diphosphate synthase from <Emphasis Type="Italic">Michelia chapensis</Emphasis> Dandy |
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Authors: | Ting Yin Xiaoying Cao Qian Miao Changgen Li Xianhui Chen Min Zhou Jihong Jiang |
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Institution: | (1) Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou, 221116, People’s Republic of China; |
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Abstract: | Farnesyl diphosphate synthase (FPS; EC 2.5.1.1/EC 2.5.1.10) catalyzes the synthesis of farnesyl diphosphate, a key intermediate
in the biosynthesis of sesquiterpenes. This present study described the cloning and characterization of a cDNA encoding FPS
from leaves of Michelia chapensis Dandy (designated as McFPS, GenBank accession number: GQ214406) for the first time. McFPS was 1,432 bp and contained an open reading frame (ORF) of 1,056 bp, encoding a protein of 351 amino acids with a calculated
molecular mass of 40.52 kDa. Bioinformatic analysis revealed that the deduced McFPS had high homology with FPSs from other
plant species. Phylogenetic tree analysis indicated that McFPS belonged to the plant FPS group and had the closest relationship
with FPS from Chimonanthus praecox. Southern blot analysis revealed that there were at most two copies of McFPS gene existed in M. chapensis genome. The organ expression pattern analysis showed that McFPS expressed strongly only in leaves, and there were no expression in stems and roots, implying that McFPS was an organ-specific expressing gene. Functional complementation of McFPS in a FPS-deficient yeast strain demonstrated that cloned cDNA encoded a farnesyl diphosphate synthase. |
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