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Maximizing okadaic acid content fromProrocentrum hoffmannianum Faust |
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| Authors: | Steve L Morton Jeffery W Bomber |
| Institution: | (1) Dept. of Plant Biology, Southern Illinois University, Carbondale, IL 62901, USA;(2) Maritech, Inc, 150 E. Pleasant Hill Rd, Carbondale, IL 62901, USA;(3) Present address: W. W. Knight Family Practice Center, 2051 Central Ave, Toledo, OH 43606, USA |
| Abstract: | Clonal cultures ofProrocentrum hoffmannianum Faust (clone 882a) were grown under optimal environmental conditions for maximal okadaic acid production. The environmental conditions of 25 °C and 86 µmol photon m-2 s-1 were used to cultivateP. hoffmannianum in a semi-continuous 36-L culture vessel with continuous cell suspension and pH control. Using these conditions, a 3-fold increase in harvestable biomass and okadaic acid content was observed when compared to batch culture techniques.Author for correspondence |
| Keywords: | Prorocentrum semi-continuous culture okadaic acid growth suspension culture |
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