中国对虾一种球状病毒的分离提纯及其核酸蛋白特性的研究

从感染致病的中国对虾（Ｐｅｎａｅｕｓｃｈｉｎｅｓｉｓ）中分离到一种球状病毒，其育径约为２０±４ｎｍ。进行人工感染实验，对虾死亡率为６６％；用脱氧核糖核酸酶（ＤＮａｓｅ），核糖核酸酶（ＲＮａｓｅ）及二苯胺染色法对病毒核酸进行处理，证明该病毒核酸为脱氧核糖核酸，用Ｓｌ核酸酶（Ｓｌｎｕｃｌｅａｓｃ）对该核酸进一步消化处理，进一步证实该病毒含单链ＤＮＡ。ＳＤＳ－ＰＡＧＥ结果显示，该病毒含４条结构多肽，其分子量分别为８６ｋＤ，７９ｋＤ，７０ｋＤ及２５．５ｋＤ。根据上述特性分析，该病毒可能属于细小病毒科（ｐａｒ－ｖｏｖｉｒｉｄａｅ），故暂定名为中国对虾细小病毒（ＰｅｎａｅｕｓｃｈｉｎｅｓｉｓＰａｒｖｏｖｉｒｕｓ简称ＰｃＰＶ）。

Isolation,Purification of Penaeus chinesis Parvovirus and Analysis of its Nucleic Acid and Protein

mall spherical virus with diameter of ca,20 nm was isolated from infected shrimps.Thevirions were icosahedron and naked. The infection exDeriment showed that the mortality was upto 66%.The viral nucleic acid was ssDNA after digested with DNase,RNase and Sl nuclease,Analysis of viral polypeptides by SDS-PAGE revealed that the virion had four structural polypep-tides with MW 86 kD,79kD,70 kD and 25.5 kD.These resul ts were in comformity with Par-voviridae,thus it was proposed that the virus be named Penaeus chinesis Parvovirus.