| 用对流电泳提纯兔出血症病毒 |
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| 引用本文： | 施卫文,杜念兴.用对流电泳提纯兔出血症病毒[J].Virologica Sinica,1995,10(3):261-264. |
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| 作者姓名： | 施卫文 杜念兴 |
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| 作者单位： | 南京农业大学动物医学院 |
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| 摘 要： | 兔出血症病毒（ＲＨＤＶ）在ｐＨ８．６的琼脂糖凝胶上电泳，在负极侧有一血凝峰，免疫电镜观察有大硅ＲＨＤＶ颗粒，证实ＲＨＤＶ病毒粒子带正电荷。利用这一特性可用对流电泳提纯病毒。将抗体与病毒颗粒形成的沉淀带切下，作ＳＤＳ－ＰＡＧＥ，经免疫转印出现６条区带，其中６０ｋＤ的ＶＰ_１为主要结构多肽。用免疫复合物提取核酸，以狄高辛标记制成探针，能与病毒核酸和克隆的ＲＨＤＶｃＤＮＡ２．２ｋｂ片段和４．８ｋｂ片段杂交，探针灵敏度达ｐｇ水平。能用病毒核酸作模板制备探针，证实ＲＨＤＶ的核酸为ＤＮＡ。
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| 关 键 词： | 兔出血症病毒 对流电泳 提纯 |
Purification of Rabbit Haemorrhagic Diseaes Virus from Counter Immunoelectropboresis |
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| Shi Weiweng ,Du Nianxing.Purification of Rabbit Haemorrhagic Diseaes Virus from Counter Immunoelectropboresis[J].中国病毒学(英文版),1995,10(3):261-264. |
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| Authors: | Shi Weiweng Du Nianxing |
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| Abstract: | HDV could be moved to the negative electrode by electrophoresis in the condition ofpH8.6 ,and this was confirmed by immunoelectron microscopy observation. The RHDV im-munocomplex was purified by counter immunoelectrophoresis(CIE)form liver tissue of rab-bits died of RHD and used for the analysis of RHDV structural polypeptide by immunoblot-ing.The RHDV nucleic acid was extracted from RHDV immunocomplex and labelled withDigoxigenin-dUTP. The labelled nucleic acid probe with high senseitivity and specificity wasable to hybrideized with RHDV nucleic acid,RHDV 2. 2kb and4. 8kb cDNA fragments in dotblot analysis. |
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| Keywords: | RHDV CIE Immunobloting Nucleic acid probe |
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