以原生质体融合技术构建广谱抗噬菌体菌株

利用１５株不同品系的噬菌体作筛子筛选的噬菌体抗性自发突变株只对其相应的筛于噬菌体具抗性，为窄谱抗株。利用原生质体融合技术把北京棒杆菌７３３８与钝齿棒杆菌Ｔｓ－Ｌｉ进行融合得融合于ＺＧ８８，通过噬菌体吸附试验，血清学试验证明ＺＧ８８细胞表面结构发生了较大的改变，失去了噬菌体的吸附位点。因此ＺＧ８８是广谱稳定的噬菌体抗性菌株。

A Broad Spectrum Antiphage Strain Constituted with Protoplast Fusion Technology

ntiphage mutants selected by 15 phage strains have resistant only to its relevant phage,50it is narrow-spectrum antiphage strain.The fusant ZG88 is obtained with protoplast fusion be-tween Corynebacterium pekinense 7338 and Corynebacterium crenatum Ts-Li.It s serologic reac-tion and phage attach test show that it has changed so much in cell surface structures as to losethe position of adsorption.So ZG 88 is a broad spectrum and steady antiphage strain.