| 侵染菊花的黄瓜花叶病毒的初步鉴定和血清学检测 |
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| 引用本文： | 张海保,朱西儒.侵染菊花的黄瓜花叶病毒的初步鉴定和血清学检测[J].Virologica Sinica,1995,10(4):367-370. |
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| 作者姓名： | 张海保 朱西儒 |
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| 作者单位： | 中国科学院华南植物研究所 |
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| 基金项目： | 广东省科委自然科学基金 |
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| 摘 要： | 侵染菊花的黄瓜花叶病毒的初步鉴定和血清学检测张海保，朱西儒，张云开（中国科学院华南植物研究所广州５１０６５０）关键词菊花，黄瓜花叶病毒，间接ＥＬＩＳＡ菊花（Ｃｈｒａｙｓａｎｔｈｅｍｕｍｍｏｒｉｆｏｌｉｕｍ）病毒病是危害菊花的一类主要病害。国外文献报道...
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| 关 键 词： | 菊花 黄瓜花叶病毒 鉴定 血清学检测 植物病毒 |
Preliminary Identification and Serological Detection of Cucumber Mosaic Virus Infecting Chrysanthemum |
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| Zhang Haibao,Zhu Xiru,Zhang Yunkai.Preliminary Identification and Serological Detection of Cucumber Mosaic Virus Infecting Chrysanthemum[J].中国病毒学(英文版),1995,10(4):367-370. |
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| Authors: | Zhang Haibao Zhu Xiru Zhang Yunkai |
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| Abstract: | he virus isolate S_1 was isolated from chrysanthemum plants with the symtoms of viral dis-ease,The host range of isolate S_1 was wide.It could infect 11 species plants aniong tested 6 fami-lies by sap-inoculation, The isulate S_1 was also transmitted in non-persistant manner by Myzuspersicae,The rate of transmission was 86.7%(13/15).The vlrus particle of isolate S_1 wassphere stiape, ItS diameter was about 28-30nm,The serological test showed that isolate S_1 re-acted positively with the antiserum against CMV but negatively with ToAV antiserum.On thebasis of these characteristics,isolate S1 was identified as a member of cucumber mosaic virus(CMV),The rabbit antiserum against CMV(isolate S1)was prepared with purified isolate S1.The highest titer of the antiserum was 1:2400 tested by ELISA.All 158 virus disease samples of field chrysanthemum plants were detected with the prepared antiserum by indirect-ELISA,inwhich 19.0% amples showed positive reaction. |
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| Keywords: | Chrysanthemum Cucumber mosaic virus(CMV) Indirect-ELISA |
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