Use of Yarrowia lipolytica hexokinase for the quantitative determination of trehalose 6-phosphate |
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Authors: | Miguel A Blázquez Juana M Gancedo Carlos Gancedo |
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Institution: | Instituto de Investigaciones Biomédicas C.S.I.C., Arturo Duperier no. 4., E-28029 Madrid, Spain |
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Abstract: | Abstract This paper describes a procedure for the quantitative determination of trehalose 6-phosphate (T6P) based on its ability to inhibit hexokinase from Yarrowia lipolytica . The assay is linear between 1 nmol and at least 8 nmol. The concentration of T6P in wild-type Saccharomyces cerevisiae (0.15 mM) and in ras2 mutants (0.25 mM) remained unchanged in the exponential or stationary phase of growth or after heat shock. A tps1 mutant affected in T6P synthase did not show detectable T6P. Heat shock increased the concentration of T6P in Schizosacharomyces pombe from 0.43 to 0.75 mM. |
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Keywords: | Trehalose 6-phosphate Yarrowia lipolytica Hexokinase Yeast |
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