睾丸去神经对大鼠半去势诱导的睾酮代偿性分泌的影响

在成年大鼠,半去势可以在促性腺激素没有明显改变的情况下导致睾丸静脉血液中睾酮浓度代偿性增加,其机理尚不明了。本研究以成年大鼠为实验动物,检验睾酮的代偿性增加是否受到睾丸去神经的影响。睾丸去神经（ｉｎｆｅｒｉｏｒ ｓｐｅｍａｔｉｃ ｎｅｒｖｅｓ,ＩＳＮ或ＩＳＮ ｐｌｕｓ ｓｕｐｅｒｉｏｒ ｓｐｅｒｍａｔｉｃ ｎｅｒｖｅｓ,ＩＳＮ－ＳＳＮ）手术２周后开始半去势实验,半去势之前和半去势之后６和２４ｈ,

Neural control of the compensatory increase of testosterone in hemicastrated adult male rats

It is unclear why the concentration of testosterone increases in the testicular vein after hemicastration without corresponding alteration in gonadotropins. The present work was undertaken to examine whether the testosterone levels could be modified by denervation of the testis in adult rats. Both systemic and testicular blood samples were collected either immediately before or 6 and 24 hours after hemicastration from the rats two weeks after denervation of either inferior spermatic nerves (ISN) or ISN plus superior spermatic nerves (ISN-SSN). Increase of testicular testosterone induced by hemicastration was significantly (P<0.05) inhibited in these rats, as compared with the sham animals (at 6 and 24 hours, ISN vs sham: 16.00+/-3.35 vs 42.72+/-13.85 and 26.93+/-8.68 vs 71.16+/-13.30 whilst ISN-SSN vs sham: 31.63+/-7.92 vs 60.61+/-18.11 and 27.70+/-8.93 vs 93.92+/-19.73 ng/ml, respectively), whereas no significant change in LH was observed in all the experimental groups. FSH underwent no alteration in all the ISN denervation groups, but a significant elevation was observed in the ISN-SSN denervation groups (P<0.05) before hemicastration. Therefore, it appears that the change in FSH is not the cause of the inhibition of testosterone increase in the hemicastrated rats after testicular denervation and that ISN plays an active role in regulation of testosterone increase induced by hemicastration.