Induction of DNA damage by free radicals generated either by organic or inorganic arsenic (AsIII, MMAIII, and DMAIII) in cultures of B and T lymphocytes |
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Authors: | Saul Espinosa Gómez Luz Maria del Razo Jose Luis Muñoz Sanchez |
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Institution: | (1) Department of Biochemistry, National School of Biological Sciences (ENCB), C.P. 11340 IPN. Carpio y Plan de Ayala S/N, Mexico;(2) Department of Toxicology, Center for Research of Advanced Studies (Cinvestav), Av IPN #2508, CP. 07360 Col. San Pedro Zacatenco, Mexico |
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Abstract: | The aim of this work is based in the premise that inorganic arsenic (AsIII) and trivalentmethylated metabolites monomethylarsonous (MMAIII) and dimethylarsinous (DMAIII) participate in DNA damage through the generation of reactive oxygen species (ROS). We have utilized two lymphoblastic lines,
Raji (B cells) and Jurkat (T cells), which were treated with the trivalent arsenic species (dose: 0–100 μM) and analyzed by two assays (comet assay and flow cytometry) in the determination of DNA damage and ROS effects in vivo.
The results showed that the damage to the DNA and the generation of ROS are different in both cellular lines with respect
to the dose of organic arsenic, and the order of damage is MMAIII>DMAIII>AsIII. This fact suggests that the DMAIII is not always the more cytotoxic intermediary xenobiotic, as has already been reported in another study. |
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Keywords: | DNA damage trivalent areenic reactive oxygen species (ROS) comet assay dihydrorhodamine 123 (DHR123) flow cytometry |
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