Phosphorylation of phospholipase B in the plasma membrane of Saccharomyces cerevisiae |
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Authors: | W Witt P Wille GF Fuhrmann |
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Institution: | Institut für Pharmakologie und Toxikologie, Philipps-Universität, Lahnberge, 3550 Marburg, F.R.G. |
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Abstract: | Abstract Plasma membrane vesicles from Saccharomyces cerevisiae were incubated with γ-32P]ATP. Several phosphorylated protein bands were separated by LiDS polyacrylamide gel electrophoresis. One of these bands with an apparent M r of 145 000 was identified by immunoprecipitation as a membrane-bound phospholipase. |
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Keywords: | Yeast phosphorylation of membrane proteins immunoprecipitation protein A LiDS polyacrylamide gel electrophoresis |
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