Immune-suppressive activity of punicalagin via inhibition of NFAT activation |
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Authors: | Lee Sang-Ik Kim Byoung-Soo Kim Kyoung-Shin Lee Samkeun Shin Kwang-Soo Lim Jong-Soon |
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Affiliation: | a Institute of Traditional Medicine and Bioscience, Daejeon University, Yongun-dong, Dong-gu, Daejeon 300-716, Republic of Korea b Department of Applied Chemistry, Daejeon University, Yongun-dong, Dong-gu, Daejeon 300-716, Republic of Korea c Department of Microbiology and Biotechnology, Daejeon University, Yongun-dong, Dong-gu, Daejeon 300-716, Republic of Korea |
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Abstract: | Since T cell activation is central to the development of autoimmune diseases, we screened a natural product library comprising 1400 samples of medicinal herbal extracts, to identify compounds that suppress T cell activity. Punicalagin (PCG) isolated from the fruit of Punica granatum was identified as a potent immune suppressant, based on its inhibitory action on the activation of the nuclear factor of activated T cells (NFAT). PCG downregulated the mRNA and soluble protein expression of interleukin-2 from anti-CD3/anti-CD28-stimulated murine splenic CD4+ T cells and suppressed mixed leukocytes reaction (MLR) without exhibiting cytotoxicity to the cells. In vivo, the PCG treatment inhibited phorbol 12-myristate 13-acetate (PMA)-induced chronic ear edema in mice and decreased CD3+ T cell infiltration of the inflamed tissue. These results suggest that PCG could be a potential candidate for the therapeutics of various immune pathologies. |
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Keywords: | Punicalagin Natural product library Immune suppressant Interleukin-2 Nuclear factor of activated T cells Mixed leukocytes reaction PMA-induced ear edema |
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