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Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein |
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| Authors: | Noisakran Sansanee Sengsai Suchada Thongboonkerd Visith Kanlaya Rattiyaporn Sinchaikul Supachok Chen Shui-Tein Puttikhunt Chunya Kasinrerk Watchara Limjindaporn Thawornchai Wongwiwat Wiyada Malasit Prida Yenchitsomanus Pa-thai |
| Institution: | a Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand b Medical Molecular Biology Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Adulyadejvikrom Building (12th Floor), Mahidol University, Bangkok 10700, Thailand c Medical Proteomics Unit, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand d Institute of Biological Chemistry and Genomic Research Center, Academia Sinica, Taipei, Taiwan e Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan f Department of Clinical Immunology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand |
| Abstract: | Dengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells. |
| Keywords: | hnRNP C1/C2 Dengue virus NS1 Interacting protein Host and viral protein interaction Virus replication |
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