Cytoskeletal pattern changes during branch formation in a centrifugedAdiantum protonema |
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Authors: | Masamitsu Wada Kazunari Nozue Akeo Kadota |
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Institution: | (1) Biology Department, Faculty of Science, Tokyo Metropolitan University, Minami Osawa, Hachioji, 192-03 Tokyo, Japan |
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Abstract: | A protonemal branch was induced on a side wall of a fern filamentous protonema by cell centrifugation and subsequent polarized-red
light irradiation as described in a previous paper (Wada 1995, J. Plant Res. 108: 501–509). Changes in microtubule (MT) and
microfilament (MF) patters during the branch development were observed under fluorescence microscopy. A ring-like band of
cortical MTs (MT-ring) and MFs similar to a preprophase band or a subapical ring structure (Murataet al. 1987) appeared transiently at the future branching site before cell swelling, the first visible step of branch formation.
At this stage, the nucleus was located far from the branching site and the MT-ring appeared to be connected to the nucleus
by endoplasmic MFs as well as with endoplasmic MTs. The MT-ring disappeared when cell wall swelling occurred. When the cell
wall swelling began, a fan-like pattern of cortical MTs emanating from the new growing tip was established and the MTs reached
the opposite flank of the protonema. When a new branch started to elongate and the nucleus moved into the branch, a faint
subapical ring of MTs appeared at the subapical part of the new branch. Strands of MTs and MFs emanating from the nuclear
front end reached a part of the subapical ring. |
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Keywords: | Adiantum Branching Cytoskeleton Microfilament Microtubule Protonema (ferm) |
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