Characterization of natural peptide ligands from HLA-DP2: new insights into HLA-DP peptide-binding motifs |
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| Authors: | Gema Díaz Benito Cañas Jesús Vazquez César Nombela Javier Arroyo |
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| Institution: | (1) Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain;(2) Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain;(3) Present address: Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, 28922 Madrid, Spain;(4) Present address: Facultad de Ciencias Químicas, Universidad Complutense de Madrid, 28040 Madrid, Spain |
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| Abstract: | Although natural peptide ligands of HLA-DR and HLA-DQ molecules have been extensively studied, information about peptides naturally bound to HLA-DP is limited. Here we describe HLA-DP2 peptide ligands corresponding to 24 different source proteins that were identified by peptide pool elution and mass spectrometry sequencing from HLA-DP2 molecules expressed on EBV-LCLs. Sequencing analysis led to the identification of both promiscuous and allele-specific peptides. Moreover, the alignment of the natural ligands for HLA-DP2 described here, combined with previous results from our group and others concerning HLA-DP2 antigen presentation and HLA-DP molecular modelling, provide a better understanding of HLA-DP2 peptide-binding motifs. |
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| Keywords: | HLA-DP antigens Polymorphism Peptide-binding HLA ligand Peptide motifs |
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