Phytohormone-induced GABA production in transformed root cultures of Datura stramonium: an in vivo 15N NMR study |
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Authors: | Ford, Y-Y. Ratcllffe, R.G. Robins, R.J. |
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Abstract: | Primary nitrogen metabolism in transformed root cultures ofDatura stramonium was observed by in vivo 15N NMR. Treatmentof the root cultures with the plant growth regulators -naphthaleneaceticacid (NAA) and kinetin caused a de-differentiation of the roottissue, together with perturbation of primary and secondarynitrogen metabolism. The levels of newly-synthesized glutamineand glutamate during ammonium assimilation were depleted relativeto control cultures, whereas GABA biosynthesis was enhanced.Although GABA production could be stimulated by a decrease incytoplasmic pH (whether imposed artificially or induced by hypoxia),observation of the roots during phytohormone treatment by 31PNMR showed that the cytoplasmic pH remained stable, indicatingthat the perturbation of nitrogen metabolism in the de-differentiatedroots must be due to other causes. Key words: Datura, -aminobutyric acid, nitrogen metabolism, NMR, root cultures |
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