Growth of human-human hybridomas in serum-free media enhances antibody secretion |
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Authors: | Mark C Glassay Robert E Peters Alexander Mikhalev |
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Institution: | (1) Biotherapeutics, Inc., 11025 N. Torrey Pines Road, 92037 La Jolla, California;(2) University of California, San Diego;(3) Department of Medicine, USCD Cancer Center, T-011, 92093 La Jolla, California;(4) Present address: Immune Response Corp., San Diego, CA |
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Abstract: | Summary Human-human hybridomas derived from fusing lymph node lymphocytes with UC 729-6 were adapted to grow in commercially available
serum-free medium and were compared with serum-supplemented 10% fetal bovine serum (FBS)] cultures. Over a 6-d period, no
significant changes occurred in the growth of the cells in 10% FBS or serum-free medium. In cultures supplemented with 10%
FBS more total proteins were secreted than in serum-free cultures. However, there was an enhanced secretion of three- to four-fold
of both immunoreactive human IgG and IgM under serum-free conditions compared to serum-supplemented conditions. Serum-free
conditions may provide the appropriate milieu for the increased level of Ig secretion from human hybridomas derived from UC
729-6 in that there are no inhibitors that may be present in serum.
The work described in this report was partially supported by grants from the National Institutes of Health (CA 32047, CA 37497),
Bethesda, MD, and the University of California Cancer Research Coordinating Committee. R.E.P. was a postdoctoral fellow supported
by the UCSD Cancer Center and M.C.G. was the recipient of an NIH New Investigator Research Award. A.M. was a visiting scientist
from the Medical Academy of Bulgaria, Sofia. |
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Keywords: | immunoglobulin UC 729-6 tumor antigens monoclonal antibodies |
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