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Selective alterations in hepatic enzyme response after reduction of nuclear triiodothyronine receptor sites by partial hepatectomy and starvation.
Authors:W H Dillmann  H L Schwartz  J H Oppenheimer
Affiliation:Molecular Biology Unit, Research School of Biological Sciences, The Australian National University, A.C.T. 2601 Australia
Abstract:Barley embryo 5S rRNA hybridizes efficiently with barley embryo 18S rRNA but not with 26S rRNA. Mouse sarcoma 5S rRNA also selectively hybridizes, to a smaller extent, with mouse sarcoma 18S rRNA. The barley embryo 5S–18S rRNA complex has a sharp melting profile and a “Tm” of ca. 59° in 0.1 M NaCl. The mouse sarcoma 5S–18S rRNA complex has a broader transition breadth and a “Tm” of ca 52°. The conditions used for hybridization lead to very specific reconstitution of the “natural” complex between 5.8S and 26S–28S rRNA since both the invivoandinvitro complexes between 5.8S and 26S–28S rRNA from barley embryos and mouse sarcoma have equally sharp melting profiles and a “Tm” of ca. 52° in 0.1 M NaCl.
Keywords:α-GPD  α-glycerophosphate dehydrogenase  malic enzyme  ME  L-3,5,3′ triiodothyronine
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