Optogenetic analysis of synaptic function |
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Authors: | Liewald Jana F Brauner Martin Stephens Greg J Bouhours Magali Schultheis Christian Zhen Mei Gottschalk Alexander |
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Affiliation: | Institute of Biochemistry, Department of Biochemistry, Chemistry and Pharmacy, Goethe University Frankfurt, Biocenter N220, Frankfurt, Germany. |
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Abstract: | We introduce optogenetic investigation of neurotransmission (OptIoN) for time-resolved and quantitative assessment of synaptic function via behavioral and electrophysiological analyses. We photo-triggered release of acetylcholine or gamma-aminobutyric acid at Caenorhabditis elegans neuromuscular junctions using targeted expression of Chlamydomonas reinhardtii Channelrhodopsin-2. In intact Channelrhodopsin-2 transgenic worms, photostimulation instantly induced body elongation (for gamma-aminobutyric acid) or contraction (for acetylcholine), which we analyzed acutely, or during sustained activation with automated image analysis, to assess synaptic efficacy. In dissected worms, photostimulation evoked neurotransmitter-specific postsynaptic currents that could be triggered repeatedly and at various frequencies. Light-evoked behaviors and postsynaptic currents were significantly (P
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