Centrifugation on a single layer of colloid selects improved quality spermatozoa from frozen-thawed stallion semen |
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| Authors: | Macías García B Morrell J M Ortega-Ferrusola C González-Fernández L Tapia J A Rodriguez-Martínez H Peña F J |
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| Institution: | Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Avda de Universidad s/n, Cáceres, Spain. |
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| Abstract: | The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post-thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane-coated silica colloid with a species-specific formulation (Androcoll-E). Sperm motility, sperm chromatin structure, membrane integrity and mitochondrial membrane potential were studied in filtered and non-filtered spermatozoa. Single-layer centrifugation (SLC) using Androcoll-E significantly improved all the sperm parameters studied, implying SLC may be a simple approach to improve the quality of frozen-thawed (FT) spermatozoa for AI. |
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