3'-modified oligo (2'-O-methylribonucleotides) as improved probes for hybridization with RNA |
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Authors: | Novopashina D S Totskaya O S Lomzov A A Venyaminova A G |
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Affiliation: | Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. danov@niboch.nsc.ru |
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Abstract: | A series of octa (2-O-methylribonucleotides) with an additional 3'-terminal deoxynucleoside (T, dC, dA or dG) linked by the 3'-3' (inverted) bond was synthesized. The exceptional stability of these oligomers to a 3'-exonuclease (SVP) and nucleases in culture medium containing 10% heat-inactivated fetal calf serum was demonstrated. It was shown that the addition of the 3'-dangling inverted deoxynucleoside increases substantially the thermal stability of the duplexes of oligo(2'-O-methylribonucleotides) with complementary RNA and DNA in the case of a relatively weak terminal AmU(T) pair and enhances the mismatch sensitivity. |
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