In vivo phosphorylation of isocitrate lyase inEscherichia coli andAcinetobacter calcoaceticus |
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Authors: | Hoyt J C Lin H -P P Reeves H C |
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Institution: | (1) Department of Microbiology, Arizona State University, 85287-2701 Tempe, Arizona, USA;(2) Present address: Department of Chemistry, Rice University, 77251 Houston, TX, USA |
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Abstract: | Isocitrate lyase inEscherichia coli and inAcinetobacter calcoaceticus is phosphorylated when the cells are grown with acetate as the sole carbon source in low-phosphate mineral salts medium containing32P inorganic phosphate. The level of32P incorporation into the enzyme in both microorganisms appears to be constant throughout the entire growth cycle. Further, theresults of immunoblots and rocket immunoelectrophoresis suggest that the amount of isocitrate lyase protein, although at different levels in each microorganism, also remains constant throughout the growth cycle. |
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