Separation of Kupffer and endothelial cells of the rat liver by centrifugal elutriation |
| |
Authors: | D. L. Knook E. Ch. Sleyster |
| |
Affiliation: | Institute for Experimental Gerontology TNO, Rijswijk, The Netherlands |
| |
Abstract: | Isolated non-parenchymal cells from rat liver were separated by centrifugal elutriation into two fractions consisting of structurally intact Kupffer and endothelial cells with purities of 91 and 95%, respectively. Purified Kupffer and endothelial cells showed nearly equal specific activities for the lysosomal enzyme acid phosphatase, whereas the specific activity of cathepsin D was about 3 times higher in Kupffer cells. It was calculated that a significant amount of the cathepsin D activity in the liver is present in the Kupffer cells. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |