Preformed and newly synthesized messenger RNA in germinating wheat embryos |
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Authors: | L. Ivo Caers Willy J. Peumans Albert R. Carlier |
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Affiliation: | (1) Laboratorium voor Plantenbiochemie, Katholieke Universiteit Leuven, Vaartstraat 24, B-3000 Leuven, Belgium |
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Abstract: | In 6 h germinated wheat (Triticum aestivum L. cv. Cama) embryos, more than half of the messenger RNAs are actively involved in translation. Neither preformed nor newly synthesized poly A+-RNA is translated preferentially. Germination in the presence of cordycepin showed that the half-life of the templates is about 2 h and that the newly synthesized messengers are essential to support protein synthesis in the embryo from the first hours of germination. Most of the messenger RNAs in 6 h germinated embryos are newly synthesized. The polypeptides coded for by either the endogenous messenger ribonucleoproteins or purified poly A+-RNA from both dry and germinated embryos are qualitatively identical; minor quantitative differences can however be observed.Abbreviations HEPES N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid - EDTA ethylenediaminetetraacetic acid - SDS sodium dodecyl sulfate - TCA trichloroacetic acid - mRNP messenger ribonucleoprotein - poly A+-RNA polyadenylic acid containing RNA - PB polysome buffer - GM germination medium |
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Keywords: | Embryo (mRNA) Germination (embryo) Messenger ribonucleoprotein mRNA Ribonucleoprotein (messenger) Translation Triticum |
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