Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus thuringiensis subsp. kurstaki |
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Authors: | Slim Tounsi Anis J'Mal Nabil Zouari Samir Jaoua |
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Institution: | (1) Centre de Biotechnologie de Sfax, Laboratoire de Protection et de Transformation des Plantes, B.P. K , 3038 Sfax, Tunisia |
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Abstract: | A newly isolated strain of B. thuringiensis, BNS3, was identified as affiliated to the subsp. kurstaki and belonging to the serotype H3a, 3b, 3c. Insecticidal crystal proteins from BNS3 were active against lepidopteran larvae, particularly Prays oleae, Ephestia kuehniella, Ostrinia nubilalis and Spodoptora exigua. The cloning and sequencing from BNS3 of a cry1Aa-type gene, called crybns3-1, revealed an open reading frame of 3531 bp, encoding a protein of 1176 amino acid residues. Both nucleotide and amino acid sequences similarity analysis revealed that crybns3-1 is a new cry1Aa-type gene, presenting several differences with the other cry1Aa-type genes. |
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Keywords: | Bacillus thuringiensis cloning cry genes delta-endotoxins DNA sequence |
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