Optimization of critical medium components using response surface methodology for biomass and extracellular polysaccharide production by <Emphasis Type="Italic">Agaricus blazei</Emphasis> |
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Authors: | Gao-Qiang Liu Xiao-Ling Wang |
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Institution: | (1) College of Life Sciences and Technology, Central South University of Forestry & Technology, 498 South Shaoshan Road, Changsha, 410004, China |
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Abstract: | Response surface methodology (RSM) was applied to optimize the critical medium ingredients of Agaricus blazei. A three-level Box–Behnken factorial design was employed to determine the maximum biomass and extracellular polysaccharide
(EPS) yields at optimum levels for glucose, yeast extract (YE), and peptone. A mathematical model was then developed to show
the effect of each medium composition and its interactions on the production of mycelial biomass and EPS. The model predicted
the maximum biomass yield of 10.86 g/l that appeared at glucose, YE, peptone of 26.3, 6.84, and 6.62 g/l, respectively, while
a maximum EPS yield of 348.4 mg/l appeared at glucose, YE, peptone of 28.4, 4.96, 5.60 g/l, respectively. These predicted
values were also verified by validation experiments. The excellent correlation between predicted and measured values of each
model justifies the validity of both the response models. The results of bioreactor fermentation also show that the optimized
culture medium enhanced both biomass (13.91 ± 0.71 g/l) and EPS (363 ± 4.1 mg/l) production by Agaricus blazei in a large-scale fermentation process. |
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Keywords: | Agaricus blazei Submerged culture Response surface methodology Box– Behnken design Extracellular polysaccharide |
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