| 沙丘芦苇特有内含物与水芦叶绿体及其蛋白质的相互作用特征 |
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| 引用本文： | 张玉霞,高松,浦铜良,刘斌斌.沙丘芦苇特有内含物与水芦叶绿体及其蛋白质的相互作用特征[J].西北植物学报,2009,29(12). |
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| 作者姓名： | 张玉霞 高松 浦铜良 刘斌斌 |
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| 作者单位： | 1. 兰州大学,生命科学学院,兰州,730000
2. 中国科学院寒区旱区环境与工程研究所,兰州,730000 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 以水生芦苇为材料,通过吸附试验、亲和试验和荧光光谱试验探讨了沙丘芦苇(Phragmites communis Trin)的特有内含物PAAC与水芦叶绿体吸附特性及其作用位点,并在固定化的条件下研究其与水芦叶片可溶性蛋白的相互作用特点,以阐明PAAC对叶绿体的保护作用机理.结果显示:(1)水芦叶绿体对PAAC的吸附符合Freundlich吸附模型,吸附量随pH的增加而增大并于pH 7.6时达到最大值(0.86 μg/μg),吸附量虽随离子强度的提高而降低,但降幅较小.(2)PAAC可与水芦可溶性蛋白结合,0.26 mol/L的NaCl溶液能够洗脱与PAAC结合的蛋白质,两者间的相互作用以盐键为主,且结合蛋白质的种类特异性不明显.(3)PAAC与水芦蛋白质之间存在着相互结合作用并可能存在变构效应,它对蛋白质的荧光猝灭作用是生成PAAC-蛋白质复合物的静态猝灭,它与蛋白质的结合常数在1.0×10~5左右及结合位点数为1∶1.
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| 关 键 词： | 叶绿体 可溶性蛋白 相互作用 吸附 |
Interaction Characteristics of Specified Contains in Dune Reed with Chloroplast and Protein of Swamp Reed |
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| ZHANG Yu-xia,GAO Song,PU Tong-liang,LIU Bin-bin.Interaction Characteristics of Specified Contains in Dune Reed with Chloroplast and Protein of Swamp Reed[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(12). |
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| Authors: | ZHANG Yu-xia GAO Song PU Tong-liang LIU Bin-bin |
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| Institution: | ZHANG Yu-xia1,GAO Song2,PU Tong-liang1,LIU Bin-bin1(1 College of Life Science,Lanzhou University,Lanzhou 730000,China,2 Cold , Arid Regions Environmental , Engineering Research Institute,Chinese Academy of Sciences,China) |
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| Abstract: | The paper studies on the adsorption characteristics and binding sites of a novel small compound specified PAAC in dune reed and chloroplasts of swamp reed as well as the interactions between PAAC and protein of swamp reed through adsorption experiment,affinity chromatography and fluorescence spectroscopy method with swamp reed material.The results indicated that the absorption of PAAC by chloroplasts suits Freundlich-model,the quantity of adsorption has a upward trend with the pH changs high.When pH=7.6,the... |
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| Keywords: | PAAC PAAC chloroplasts soluble protein interaction adsorption |
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