Microbiological assay of carnitine |
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| Authors: | L R Travassos C O Sales |
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| Institution: | 1. Departamento de Microbiologia Geral, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, GB, Brasil;2. Department of Dermatology, College of Physicians and Surgeons of Columbia University, New York, New York 10032 USA |
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| Abstract: | A method for carnitine assay has been devised by the growth response of the yeast Torulopsis bovina. As little as 0.0005 μg/ml of l (?) carnitine could be assayed by this method. Free carnitine was assayed in several biological materials together with the carnitine present as soluble esters. Lipid-bound carnitine was precipitated by trichloroacetic acid and assayed independently. Average recovery of added carnitine in the free-carnitine assays was 95%. In lipid-bound carnitine assays, recovery of added carnitine ranged from 76.2 to 95%. Treatment of materials for the microbiological assays included separation in ion-exchange resins and alkaline or acid hydrolyses. Materials containing negligible amounts of carnitine esters could be assayed directly without further treatment. |
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