Evaluation of the Korean isolate-1 tachyzoite antigen for serodiagnosis of toxoplasmosis |
| |
Authors: | Shin Eun-Hee Kim Dong-Hee Lin Aifen Lee Jo-Woon-Yi Kim Hyo-Jin Ahn Myoung-Hee Chai Jong-Yil |
| |
Affiliation: | Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Republic of Korea. |
| |
Abstract: | To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis. |
| |
Keywords: | Toxoplasma gondii Toxoplasmosis Korean isolate-1 (KI-1) RH strain Serodiagnosis Antigen |
本文献已被 PubMed 等数据库收录! |
|