The Ixodes scapularis salivary protein, salp15, prevents the association of HIV-1 gp120 and CD4 |
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Authors: | Juncadella Ignacio J Garg Renu Bates Tonya C Olivera Elias R Anguita Juan |
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Institution: | Department of Veterinary and Animal Sciences, University of Massachusetts Amherst, 103 Paige Laboratory, 161 Holdsworth Way, Amherst, MA 01003, USA. |
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Abstract: | Ixodes scapularis salivary protein, Salp15, inhibits CD4(+) T cell activation by binding to the most-extracellular domains of the CD4 molecule, potentially overlapping with the gp120-binding region. We now show that Salp15 inhibits the interaction of gp120 and CD4. Furthermore, Salp15 prevents syncytia formation between HL2/3 (a stable HeLa cell line expressing the envelope protein) and CD4-expressing cells. Salp15 prevented gp120-CD4 interaction at least partially through its direct interaction with the envelope glycoprotein. A phage display library screen provided the interacting residues in the C1 domain of gp120. These results provide a potential basis to define exposed gp120 epitopes for the generation of neutralizing vaccines. |
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