Leishmania tropica and Leishmania donovani: solid phase radioimmunoassay using leishmanial excreted factor |
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Authors: | J El-On U Zehavi H Avraham C L Greenblatt |
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Institution: | 1. Department of Parasitotogy and the Kuvin Centre for the Study of Infectious and Tropical Diseases, Jerusalem Israel;2. Department of Immunology, Hebrew University—Hadassah Medical School, Jerusalem Israel;3. Faculty of Agriculture, The Hebrew University, Rehovot, Israel |
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Abstract: | A radioimmunoassay for the quantitative determination of anti-leishmanial excreted factor (EF) antibody in rabbit sera was developed. The assay, using Leishmania tropica and Leishmania donovani promastigotes EF, purified by either extraction with phenol followed by fractionation on a Sephadex G-100 column or by the dissociation of EF antibody complexes, was shown to be sensitive and reproducible. Using monospecific anti-EF antibodies, levels of as low as 0.06-0.12 micrograms/ml of anti-EF IgG could be detected. The specificity of the assay was assessed by inhibition with homologous and heterologous EF. Only minor cross-reactivity with heterologous EF was observed, and as little as 2.5 micrograms/ml of EF could be detected. Sera from kala-azar patients showed only 1.8-3.1 times more anti-EF activity, as compared with uninfected controls. No specificity was observed with sera from kala-azar patients with regard to the type of EF used. Almost the same activity was obtained with both EF from L. tropica and L. donovani. No anti-EF antibodies were detected in sera from patients with cutaneous leishmaniasis. |
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Keywords: | Protozoa parasitic Excreted factor EF Radioimmunoassay Leishmaniasis cutaneous Kala-azar |
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