Cell-based chemical genetic screen identifies damnacanthal as an inhibitor of HIV-1 Vpr induced cell death |
| |
Authors: | Kamata Masakazu Wu Raymond P An Dong Sung Saxe Jonathan P Damoiseaux Robert Phelps Michael E Huang Jing Chen Irvin S Y |
| |
Affiliation: | Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA. masa3k@ucla.edu |
| |
Abstract: | Viral protein R (Vpr), one of the human immunodeficiency virus type 1 (HIV-1) accessory proteins, contributes to multiple cytopathic effects, G2 cell cycle arrest and apoptosis. The mechanisms of Vpr have been intensely studied because it is believed that they underlie HIV-1 pathogenesis. We here report a cell-based small molecule screen on Vpr induced cell death in the context of HIV-1 infection. From the screen of 504 bioactive compounds, we identified damnacanthal (Dam), a component of noni [corrected] as an inhibitor of Vpr induced cell death. Our studies illustrate a novel efficient platform for drug discovery and development in anti-HIV therapy which should also be applicable to other viruses. |
| |
Keywords: | HIV-1 Vpr Screening Damnacanthal Apoptosis G2 arrest |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|