Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures |
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Authors: | Panitch Boonsnongcheep Sirintra Korsangruang Noppamas Soonthornchareonnon Yupyn Chintapakorn Promchit Saralamp and Sompop Prathanturarug |
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Institution: | (1) Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-ayuthaya Road, Bangkok, 10400, Thailand;(2) Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-ayuthaya Road, Bangkok, 10400, Thailand;(3) Department of Botany, Faculty of Science, Chulalongkorn University, Phayathai Road, Bangkok, 10330, Thailand; |
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Abstract: | We established cell suspension cultures derived from leaf, stem, and root calli of Pueraria candollei var. candollei and P. candollei var. mirifica using liquid Murashige and Skoog (MS) medium supplemented with 0.56 μM 6-benzyladenine (BA) and 4.52 μM 2,4-dichlorophenoxyacetic
acid (2,4-D). Growth of the cell suspension cultures progressed to the stationary phase within 15–24 days. Methanolic extracts
of cell suspension cultures of both varieties of P. candollei were analyzed using a validated HPLC protocol. All cell lines derived from leaf, stem, and root explants produced four major
isoflavonoids: daidzein, daidzin, genistein, and genistin; these isoflavonoids were detected only in the roots of intact plants.
Furthermore, the isoflavonoid contents of the cell suspension cultures were higher than those of intact plants. Thus, cell
suspension culture of both varieties of P. candollei may be an effective tool for isoflavonoid production. |
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