Isolation and characterization of a novel glycogen synthase kinase-3 gene, <Emphasis Type="Italic">GmGSK</Emphasis>, in <Emphasis Type="Italic">Glycine max</Emphasis> L. that enhances abiotic stress tolerance in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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Authors: | Chunbao Zhang Hongkun Zhao Yanzhi Liu Qiyun Li Xiaodong Liu Hua Tan Cuiping Yuan Yingshan Dong |
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Institution: | (1) College of Life Science, Northeast Normal University, 130024 Changchun, People’s Republic of China;(2) Biotechnology Research Center, Jilin Academy of Agricultural Sciences, 130033 Changchun, People’s Republic of China; |
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Abstract: | A novel glycogen synthase kinase-3 gene, GmGSK, was isolated from Glycine
max. It is 1,596 bp in length with one ORF of 410 amino acids. Southern blot analysis revealed that it has at least two copies
in the G. max genome. GmGSK, when transiently expressed in Nicotiana tabacum leaves, was localized in both cell membrane and cytoplasm. Northern blot analysis indicated that GmGSK is expressed in all tissues, with highest expression in the root. GmGSK can be induced by various abiotic stresses. When transformed with GmGSK, Saccharomyces cerevisiae exhibited enhanced resistance to salt and drought stress. |
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